Antibody Ins and Outs

Antibody Ins and Outs

Learn about the importance of antibodies and how their different qualities can impact your experiments.

About this course

Antibodies play a large role in Western blotting. This only increases the importance of choosing the correct antibodies for your experiment. This course will explain the various details of both primary and secondary antibodies, giving you a firm foundation for any antibody work in your future research.

Curriculum24 min

  • General Information
  • Preview
    Key Terms 3 min
  • What Are Antibodies? 5 min

    Learn about antibody structure and the difference between affinity and avidity.

  • Antibodies in the Western Blot Process 2 min

    Look at the Western blot workflow and where antibodies play a crucial role. 

  • Primary Antibodies
  • Polyclonal Antibodies 3 min

    Learn how polyclonal antibodies are made and how that leads to their advantages and disadvantages.

  • Monoclonal Antibodies 2 min

    Learn how monoclonal antibodies differ from polyclonal antibodies, and how that leads to their advantages and disadvantages.

  • Recombinant Antibodies 2 min

    Learn how recombinant antibodies are made and how that leads to their advantages and disadvantages.

  • Primary Antibody Comparison 1 min

    Compare the different types of primary antibodies to find the one that fits your experiment best.

  • Secondary Antibodies
  • Secondary Antibodies 2 min

    Learn about the basic characteristics of a secondary antibody, including clonality and labeling options. 

  • Course Summary 1 min

    A summary of the information learned within the course. 

  • Return to the Homepage

About this course

Antibodies play a large role in Western blotting. This only increases the importance of choosing the correct antibodies for your experiment. This course will explain the various details of both primary and secondary antibodies, giving you a firm foundation for any antibody work in your future research.

Curriculum24 min

  • General Information
  • Preview
    Key Terms 3 min
  • What Are Antibodies? 5 min

    Learn about antibody structure and the difference between affinity and avidity.

  • Antibodies in the Western Blot Process 2 min

    Look at the Western blot workflow and where antibodies play a crucial role. 

  • Primary Antibodies
  • Polyclonal Antibodies 3 min

    Learn how polyclonal antibodies are made and how that leads to their advantages and disadvantages.

  • Monoclonal Antibodies 2 min

    Learn how monoclonal antibodies differ from polyclonal antibodies, and how that leads to their advantages and disadvantages.

  • Recombinant Antibodies 2 min

    Learn how recombinant antibodies are made and how that leads to their advantages and disadvantages.

  • Primary Antibody Comparison 1 min

    Compare the different types of primary antibodies to find the one that fits your experiment best.

  • Secondary Antibodies
  • Secondary Antibodies 2 min

    Learn about the basic characteristics of a secondary antibody, including clonality and labeling options. 

  • Course Summary 1 min

    A summary of the information learned within the course. 

  • Return to the Homepage