Estimating Protein Concentration

Estimating Protein Concentration

Learn about the importance of estimating your protein concentration and how to incorporate this technique into your experimental design.

About this course

As scientists who perform Western blots all the time, we all know that the process takes time. So, why do we have to estimate our protein concentration before every Western blot? This course explores that question and more while going into detail on how protein concentration can affect your assay and even the conclusions that you draw from your results. 

Curriculum38 min

  • Importance of Estimating Protein Concentration 3 min
  • Preview
    What is a Protein Assay? 4 min
  • Science Behind Protein Assays 3 min
  • Spectrophotometry 3 min
  • Ultraviolet Absorption Spectroscopy 3 min
  • Bradford Assay 5 min
  • Lowry Assay 5 min
  • BCA Assay 5 min
  • Best Practices 4 min
  • Next Steps 3 min
  • Return to the Homepage

About this course

As scientists who perform Western blots all the time, we all know that the process takes time. So, why do we have to estimate our protein concentration before every Western blot? This course explores that question and more while going into detail on how protein concentration can affect your assay and even the conclusions that you draw from your results. 

Curriculum38 min

  • Importance of Estimating Protein Concentration 3 min
  • Preview
    What is a Protein Assay? 4 min
  • Science Behind Protein Assays 3 min
  • Spectrophotometry 3 min
  • Ultraviolet Absorption Spectroscopy 3 min
  • Bradford Assay 5 min
  • Lowry Assay 5 min
  • BCA Assay 5 min
  • Best Practices 4 min
  • Next Steps 3 min
  • Return to the Homepage