Sample Preparation Through Drying the Membrane

Sample Preparation Through Drying the Membrane

Learn about the basics of Western blotting from multiple angles, including the science and best practices behind the technique.

Description

Whether you are a Western blotting novice or an expert returning for a quick refresher, this online training series will guide you through the basic Western blotting steps. You will learn how to minimize error and get the most accurate, consistent results.

Each tutorial includes information on the science behind each Western blotting step, as well as the step-wise procedures. You will also learn some best practices for getting repeatable Western blots, so that you have confidence in your results.

  • Sample preparation
  • Gel preparation
  • Equilibrating the gel prior to transfer
  • Preparing for transfer
  • Transferring the proteins
  • Drying the membrane

Course Release Date: October 25, 2017

Curriculum52 min

  • Welcome to Lambda U 1 min
  • What is a Western Blot? 3 min
  • Sample Preparation
  • Sample Preparation: Science 2 min
  • Sample Preparation: Best Practices 1 min
  • Sample Preparation: Mechanics 2 min
  • Gel Preparation
  • Gel Preparation: Science 4 min
  • Gel Preparation: Best Practices 1 min
  • Gel Preparation: Mechanics 5 min
  • Equilibrating the Gel
  • Equilibrating the Gel: Science 2 min
  • Equilibrating the Gel: Best Practices 1 min
  • Equilibrating the Gel: Mechanics 2 min
  • Preparing for Transfer
  • Preparing for Transfer: Science 4 min
  • Preparing for Transfer: Best Practices 1 min
  • Preparing for Transfer: Mechanics 2 min
  • Transferring the Proteins
  • Transferring the Proteins: Science 2 min
  • Transferring the Proteins: Best Practices 2 min
  • Transferring the Proteins: Mechanics 4 min
  • Drying the Membrane
  • Drying the Membrane: Science 1 min
  • Drying the Membrane: Best Practices 1 min
  • Drying the Membrane: Mechanics 2 min
  • Keep Your Work Environment Clean
  • Keep Your Work Environment Clean: Best Practices 1 min
  • Feedback - Let Us Know Your Thoughts
  • Return to the Homepage

Description

Whether you are a Western blotting novice or an expert returning for a quick refresher, this online training series will guide you through the basic Western blotting steps. You will learn how to minimize error and get the most accurate, consistent results.

Each tutorial includes information on the science behind each Western blotting step, as well as the step-wise procedures. You will also learn some best practices for getting repeatable Western blots, so that you have confidence in your results.

  • Sample preparation
  • Gel preparation
  • Equilibrating the gel prior to transfer
  • Preparing for transfer
  • Transferring the proteins
  • Drying the membrane

Course Release Date: October 25, 2017

Curriculum52 min

  • Welcome to Lambda U 1 min
  • What is a Western Blot? 3 min
  • Sample Preparation
  • Sample Preparation: Science 2 min
  • Sample Preparation: Best Practices 1 min
  • Sample Preparation: Mechanics 2 min
  • Gel Preparation
  • Gel Preparation: Science 4 min
  • Gel Preparation: Best Practices 1 min
  • Gel Preparation: Mechanics 5 min
  • Equilibrating the Gel
  • Equilibrating the Gel: Science 2 min
  • Equilibrating the Gel: Best Practices 1 min
  • Equilibrating the Gel: Mechanics 2 min
  • Preparing for Transfer
  • Preparing for Transfer: Science 4 min
  • Preparing for Transfer: Best Practices 1 min
  • Preparing for Transfer: Mechanics 2 min
  • Transferring the Proteins
  • Transferring the Proteins: Science 2 min
  • Transferring the Proteins: Best Practices 2 min
  • Transferring the Proteins: Mechanics 4 min
  • Drying the Membrane
  • Drying the Membrane: Science 1 min
  • Drying the Membrane: Best Practices 1 min
  • Drying the Membrane: Mechanics 2 min
  • Keep Your Work Environment Clean
  • Keep Your Work Environment Clean: Best Practices 1 min
  • Feedback - Let Us Know Your Thoughts
  • Return to the Homepage