Blocking Step Through Image Adjustment for NIR

Blocking Step Through Image Adjustment for NIR

Learn about the basics of Near-Infrared Western blotting, including blocking the membrane, antibody selection, and more.

About this course

This course focuses on using near-infrared fluorescence detection for your Western blots. The lessons will guide you through the steps involved in blocking the membrane, dilution of primary and secondary antibodies, and imaging your Western blot membrane using near-infrared detection. 

Sections are broken down into short, easy-to-follow steps demonstrating best practices and providing tips that can significantly improve the quality and consistency of your near-infrared fluorescence Western blots.

Course Release Date: October 25, 2017

Curriculum25 min

  • Introduction to Near-Infrared Western Blotting 1 min
  • NIR: Blocking Buffer
  • Blocking Buffer: Science 1 min
  • Blocking Buffer: Best Practices 1 min
  • Blocking Buffer: Mechanics 2 min
  • NIR: Primary Antibody
  • Primary Antibody: Science 2 min
  • Primary Antibody: Best Practices 1 min
  • Primary Antibody: Mechanics 2 min
  • NIR: Washing
  • Importance of Washing 1 min
  • NIR: Secondary Antibody
  • Secondary Antibody: Science 2 min
  • Secondary Antibody: Best Practices 1 min
  • Secondary Antibody: Mechanics 2 min
  • NIR: Detection
  • Detection: Science and Best Practices 1 min
  • Detection: Mechanics 1 min
  • General: Image Adjustment
  • Image Adjustment: Best Practices 4 min
  • Return to the Homepage

About this course

This course focuses on using near-infrared fluorescence detection for your Western blots. The lessons will guide you through the steps involved in blocking the membrane, dilution of primary and secondary antibodies, and imaging your Western blot membrane using near-infrared detection. 

Sections are broken down into short, easy-to-follow steps demonstrating best practices and providing tips that can significantly improve the quality and consistency of your near-infrared fluorescence Western blots.

Course Release Date: October 25, 2017

Curriculum25 min

  • Introduction to Near-Infrared Western Blotting 1 min
  • NIR: Blocking Buffer
  • Blocking Buffer: Science 1 min
  • Blocking Buffer: Best Practices 1 min
  • Blocking Buffer: Mechanics 2 min
  • NIR: Primary Antibody
  • Primary Antibody: Science 2 min
  • Primary Antibody: Best Practices 1 min
  • Primary Antibody: Mechanics 2 min
  • NIR: Washing
  • Importance of Washing 1 min
  • NIR: Secondary Antibody
  • Secondary Antibody: Science 2 min
  • Secondary Antibody: Best Practices 1 min
  • Secondary Antibody: Mechanics 2 min
  • NIR: Detection
  • Detection: Science and Best Practices 1 min
  • Detection: Mechanics 1 min
  • General: Image Adjustment
  • Image Adjustment: Best Practices 4 min
  • Return to the Homepage