Linear Range for Quantitative Western Blots

Linear Range for Quantitative Western Blots

Learn more about what linear range and dynamic range are, why they are important, and how they play a significant role in getting high quality data that you can count on.

About this course

In this course, you will learn the meaning of the terms dynamic range, linear range, dynamic linear range and combined linear range, how they relate to quantitative Western blot detection and normalization, and the steps to determine combined linear range for your target and internal loading control in preparation for normalizing your data.

Course launch date: April 4, 2018

Curriculum25 min

  • What is Dynamic Range 3 min
  • Dynamic Range of Different Detectors 3 min
  • What is Linear Range 2 min
  • The Role of r^2 3 min
  • Examples of Using Linear Approximation 4 min
  • Best Practices
  • Best Practices 2 min
  • Calculation Example
  • Calculating Linear Range Example 8 min
  • Calculation Exercises
  • Linear Range Exercises
  • Return to the Homepage

About this course

In this course, you will learn the meaning of the terms dynamic range, linear range, dynamic linear range and combined linear range, how they relate to quantitative Western blot detection and normalization, and the steps to determine combined linear range for your target and internal loading control in preparation for normalizing your data.

Course launch date: April 4, 2018

Curriculum25 min

  • What is Dynamic Range 3 min
  • Dynamic Range of Different Detectors 3 min
  • What is Linear Range 2 min
  • The Role of r^2 3 min
  • Examples of Using Linear Approximation 4 min
  • Best Practices
  • Best Practices 2 min
  • Calculation Example
  • Calculating Linear Range Example 8 min
  • Calculation Exercises
  • Linear Range Exercises
  • Return to the Homepage