- Details of Antibody Validation
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The Importance of Antibody Validation 3 min
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What is Validation? 2 min
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Specificity, Selectivity, and Reproducibility 4 min
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Antibody Selection Process 3 min
- Validation of Specificity
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Genetic Verification 3 min
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Multiple Independent Antibodies 3 min
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Pre-Adsorption With a Blocking Peptide 2 min
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Mass Spectrometry (MS) 1 min
- Validation of Selectivity
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Validation of Antibody Selectivity 4 min
- Validation of Reproducibility
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Validation of Reproducibility 2 min
- Applying Your Knowledge
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Antibody Validation Checklist 1 min
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Antibody Validation
Antibody validation is an important step in the Western blot process. Learn more about how specificity, selectivity, and reproducibility impact your antibody choice.
This course will cover what antibody validation is, what properties of antibodies should be tested, and how to choose and validate antibodies that fit your experimental context in the Western blot application.
Course Release Date: April 4, 2018