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Antibody Validation

Antibody Validation

Antibody validation is an important step in the Western blot process. Learn more about how specificity, selectivity, and reproducibility impact your antibody choice.

About this course

This course will cover what antibody validation is, what properties of antibodies should be tested, and how to choose and validate antibodies that fit your experimental context in the Western blot application.

Course Release Date: April 4, 2018

Curriculum28 min

  • Details of Antibody Validation
  • The Importance of Antibody Validation 3 min
  • What is Validation? 2 min
  • Specificity, Selectivity, and Reproducibility 4 min
  • Antibody Selection Process 3 min
  • Validation of Specificity
  • Genetic Verification 3 min
  • Multiple Independent Antibodies 3 min
  • Pre-Adsorption With a Blocking Peptide 2 min
  • Mass Spectrometry (MS) 1 min
  • Validation of Selectivity
  • Validation of Antibody Selectivity 4 min
  • Validation of Reproducibility
  • Validation of Reproducibility 2 min
  • Applying Your Knowledge
  • Antibody Validation Checklist 1 min
  • Return to the Homepage

About this course

This course will cover what antibody validation is, what properties of antibodies should be tested, and how to choose and validate antibodies that fit your experimental context in the Western blot application.

Course Release Date: April 4, 2018

Curriculum28 min

  • Details of Antibody Validation
  • The Importance of Antibody Validation 3 min
  • What is Validation? 2 min
  • Specificity, Selectivity, and Reproducibility 4 min
  • Antibody Selection Process 3 min
  • Validation of Specificity
  • Genetic Verification 3 min
  • Multiple Independent Antibodies 3 min
  • Pre-Adsorption With a Blocking Peptide 2 min
  • Mass Spectrometry (MS) 1 min
  • Validation of Selectivity
  • Validation of Antibody Selectivity 4 min
  • Validation of Reproducibility
  • Validation of Reproducibility 2 min
  • Applying Your Knowledge
  • Antibody Validation Checklist 1 min
  • Return to the Homepage