Chemiluminescence: Detection

Chemiluminescence: Detection

There are several things to keep in mind when using a chemiluminescent detection system. In this course, you'll learn about the importance of the substrate and how it could impact your final results, as well other considerations that will help you get consistent and robust data from your Western blo

About this course

There are several things to keep in mind when using a chemiluminescent detection system. In this course, you'll learn about the importance of the substrate and how it could impact your final results, as well other considerations that will help you get consistent and robust data from your Western blot.

Curriculum52 min

  • Science
  • ECL Detection Methods and Substrate Choices 5 min
  • Detection Protocol and Stripping the Membrane 4 min
  • Troubleshooting Your Blot 7 min
  • Best Practices
  • Importance of Controlling the Variables 5 min
  • Substrate Best Practices 8 min
  • Imaging Considerations 4 min
  • Getting the Best Data Possible For Analysis 5 min
  • Downside of Stripping and Reprobing 6 min
  • General Troubleshooting for Chemi Blots 4 min
  • In the Lab
  • Detection 4 min
  • Knowledge Check
  • Return to the Homepage

About this course

There are several things to keep in mind when using a chemiluminescent detection system. In this course, you'll learn about the importance of the substrate and how it could impact your final results, as well other considerations that will help you get consistent and robust data from your Western blot.

Curriculum52 min

  • Science
  • ECL Detection Methods and Substrate Choices 5 min
  • Detection Protocol and Stripping the Membrane 4 min
  • Troubleshooting Your Blot 7 min
  • Best Practices
  • Importance of Controlling the Variables 5 min
  • Substrate Best Practices 8 min
  • Imaging Considerations 4 min
  • Getting the Best Data Possible For Analysis 5 min
  • Downside of Stripping and Reprobing 6 min
  • General Troubleshooting for Chemi Blots 4 min
  • In the Lab
  • Detection 4 min
  • Knowledge Check
  • Return to the Homepage