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Blocking Step Through Image Adjustment for Chemiluminescence

Blocking Step Through Image Adjustment for Chemiluminescence

Learn about the basics of chemiluminescent Western blotting, including blocking the membrane, antibody selection, and more.

About this course

This part of the series is focused on using chemiluminescence for your Western blot detection. It will guide you through the steps involved in blocking the membrane, dilution of primary and secondary antibodies, and the preparation and incubation of the Western blot membrane in chemiluminescence substrate.   

Sections are broken down into short, easy-to-follow steps demonstrating best practices and providing tips that can significantly improve the quality and consistency of your chemiluminescent Western blots.

Course Release Date: October 25, 2017

Curriculum29 min

  • Introduction to Chemiluminescent Western Blotting 1 min
  • CHEMI: Blocking Buffer
  • Blocking Buffer: Science 2 min
  • Blocking Buffer: Best Practices 1 min
  • Blocking Buffer: Mechanics 2 min
  • CHEMI: Primary Antibody
  • Primary Antibody: Science 1 min
  • Primary Antibody: Best Practices 1 min
  • Primary Antibody: Mechanics 3 min
  • CHEMI: Washing
  • Importance of Washing 1 min
  • CHEMI: Secondary Antibody
  • Secondary Antibody: Science 1 min
  • Secondary Antibody: Best Practices 1 min
  • Secondary Antibody: Mechanics 1 min
  • CHEMI: Detection
  • Detection: Science 1 min
  • Detection: Best Practices 1 min
  • Detection: Mechanics 3 min
  • General: Image Adjustment
  • Image Adjustment: Best Practices 4 min
  • Return to the Homepage

About this course

This part of the series is focused on using chemiluminescence for your Western blot detection. It will guide you through the steps involved in blocking the membrane, dilution of primary and secondary antibodies, and the preparation and incubation of the Western blot membrane in chemiluminescence substrate.   

Sections are broken down into short, easy-to-follow steps demonstrating best practices and providing tips that can significantly improve the quality and consistency of your chemiluminescent Western blots.

Course Release Date: October 25, 2017

Curriculum29 min

  • Introduction to Chemiluminescent Western Blotting 1 min
  • CHEMI: Blocking Buffer
  • Blocking Buffer: Science 2 min
  • Blocking Buffer: Best Practices 1 min
  • Blocking Buffer: Mechanics 2 min
  • CHEMI: Primary Antibody
  • Primary Antibody: Science 1 min
  • Primary Antibody: Best Practices 1 min
  • Primary Antibody: Mechanics 3 min
  • CHEMI: Washing
  • Importance of Washing 1 min
  • CHEMI: Secondary Antibody
  • Secondary Antibody: Science 1 min
  • Secondary Antibody: Best Practices 1 min
  • Secondary Antibody: Mechanics 1 min
  • CHEMI: Detection
  • Detection: Science 1 min
  • Detection: Best Practices 1 min
  • Detection: Mechanics 3 min
  • General: Image Adjustment
  • Image Adjustment: Best Practices 4 min
  • Return to the Homepage