Chemiluminescence: Blocking the Membrane

Chemiluminescence: Blocking the Membrane

Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

About this course

Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Curriculum40 min

  • Overview of Chemiluminescence 3 min
  • Science
  • Importance of Blocking the Membrane 3 min
  • Available Blocking Solutions 6 min
  • Commercially Available Blockers vs. Homemade Blockers 3 min
  • Best Practices
  • Maintaining the Proteins on the Membrane 7 min
  • Rehydrating the Membrane 4 min
  • Different Blockers for Different Situations 3 min
  • Pros and Cons of the Available Blocking Buffers 8 min
  • In the Lab
  • Blocking the Membrane 3 min
  • Knowledge Check
  • Return to the Homepage

About this course

Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Curriculum40 min

  • Overview of Chemiluminescence 3 min
  • Science
  • Importance of Blocking the Membrane 3 min
  • Available Blocking Solutions 6 min
  • Commercially Available Blockers vs. Homemade Blockers 3 min
  • Best Practices
  • Maintaining the Proteins on the Membrane 7 min
  • Rehydrating the Membrane 4 min
  • Different Blockers for Different Situations 3 min
  • Pros and Cons of the Available Blocking Buffers 8 min
  • In the Lab
  • Blocking the Membrane 3 min
  • Knowledge Check
  • Return to the Homepage