NIR: Blocking the Membrane

NIR: Blocking the Membrane

NIR technology is relatively new but is a very powerful way to image Western blots. In this course, you'll learn about the blocking step in the NIR Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

About this course

NIR technology is relatively new but is a very powerful way to image Western blots. In this course, you'll learn about the blocking step in the NIR Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Curriculum41 min

  • Science
  • Overview of Near-Infrared Fluorescence 5 min
  • Importance of the Blocking Step 3 min
  • Buffer System Options 3 min
  • Choices for Blocking Buffers 7 min
  • Best Practices
  • Important Considerations for Drying and Rehydrating Your Membrane 5 min
  • Ensuring Even Blocking of Your Membrane 3 min
  • Choosing the Right Blocking Buffer 3 min
  • Available Blocking Buffer Options 5 min
  • Tips for Blocking Your Membrane 4 min
  • In the Lab
  • Blocking the Membrane 3 min
  • Knowledge Check
  • Return to the Homepage

About this course

NIR technology is relatively new but is a very powerful way to image Western blots. In this course, you'll learn about the blocking step in the NIR Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Curriculum41 min

  • Science
  • Overview of Near-Infrared Fluorescence 5 min
  • Importance of the Blocking Step 3 min
  • Buffer System Options 3 min
  • Choices for Blocking Buffers 7 min
  • Best Practices
  • Important Considerations for Drying and Rehydrating Your Membrane 5 min
  • Ensuring Even Blocking of Your Membrane 3 min
  • Choosing the Right Blocking Buffer 3 min
  • Available Blocking Buffer Options 5 min
  • Tips for Blocking Your Membrane 4 min
  • In the Lab
  • Blocking the Membrane 3 min
  • Knowledge Check
  • Return to the Homepage