Fluorescence: Primary Antibody Incubation

Fluorescence: Primary Antibody Incubation

Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots.

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About this course

Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots. 

Curriculum31 min

  • Science
  • Selecting the Best Primary Antibody 3 min
  • Primary Antibody Options 5 min
  • Primary Antibody Incubation and Washing Protocol 4 min
  • Best Practices
  • Choosing the Right Primary Antibody 4 min
  • Preparing Your Primary Antibody Solution 3 min
  • Probing Your Membrane and Multiplexing 5 min
  • Importance of Washing Your Membrane 4 min
  • In the Lab
  • Primary Antibody Incubation 3 min
  • Knowledge Check
  • Return to the Homepage

About this course

Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots. 

Curriculum31 min

  • Science
  • Selecting the Best Primary Antibody 3 min
  • Primary Antibody Options 5 min
  • Primary Antibody Incubation and Washing Protocol 4 min
  • Best Practices
  • Choosing the Right Primary Antibody 4 min
  • Preparing Your Primary Antibody Solution 3 min
  • Probing Your Membrane and Multiplexing 5 min
  • Importance of Washing Your Membrane 4 min
  • In the Lab
  • Primary Antibody Incubation 3 min
  • Knowledge Check
  • Return to the Homepage