NIR: Secondary Antibody Incubation

NIR: Secondary Antibody Incubation

Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results.

About this course

Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results. 

Curriculum31 min

  • Science
  • What are Secondary Antibodies? 3 min
  • Fluorescent Dye and Multiplexing Options 8 min
  • Secondary Antibody Incubation and Washing Protocol 4 min
  • Best Practices
  • Best Practices for Minimizing Background 4 min
  • Multiplexing 3 min
  • Preparing Your Secondary Antibody 3 min
  • Optimizing Your Secondary Antibody 3 min
  • In the Lab
  • Secondary Antibody Incubation 3 min
  • Knowledge Check
  • Return to the Homepage

About this course

Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results. 

Curriculum31 min

  • Science
  • What are Secondary Antibodies? 3 min
  • Fluorescent Dye and Multiplexing Options 8 min
  • Secondary Antibody Incubation and Washing Protocol 4 min
  • Best Practices
  • Best Practices for Minimizing Background 4 min
  • Multiplexing 3 min
  • Preparing Your Secondary Antibody 3 min
  • Optimizing Your Secondary Antibody 3 min
  • In the Lab
  • Secondary Antibody Incubation 3 min
  • Knowledge Check
  • Return to the Homepage