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Introduction to Quantitative Western Blotting (FREE)

Introduction to Quantitative Western Blotting (FREE)

Learn what a quantitative Western blot is and is not, and how it differs from a qualitative Western blot.

About this course

In this course, you will learn what a quantitative Western blot is and is not, and how it differs from a qualitative Western blot. You will also learn why quantitative Western blotting is important and for which applications this technique can be used.

Course Release Date: April 4, 2018

Curriculum19 min

  • Types of Western Blots
  • Introduction to Qualitative Western Blots 3 min
  • Semi-Quantitative Western Blots 2 min
  • Quantitative Western Blots 3 min
  • Basic Criteria for Quantitative Western Blotting
  • Validate Your Antibodies 2 min
  • Include An Internal Reference Signal 2 min
  • Quantify Within the Combined Linear Range 2 min
  • Use the Appropriate Detection Chemistry 1 min
  • Use An Appropriate Imaging Platform and Analysis Software 2 min
  • Include Biological and Technical Replicates 2 min
  • Test Your Knowledge
  • Return to the Homepage

About this course

In this course, you will learn what a quantitative Western blot is and is not, and how it differs from a qualitative Western blot. You will also learn why quantitative Western blotting is important and for which applications this technique can be used.

Course Release Date: April 4, 2018

Curriculum19 min

  • Types of Western Blots
  • Introduction to Qualitative Western Blots 3 min
  • Semi-Quantitative Western Blots 2 min
  • Quantitative Western Blots 3 min
  • Basic Criteria for Quantitative Western Blotting
  • Validate Your Antibodies 2 min
  • Include An Internal Reference Signal 2 min
  • Quantify Within the Combined Linear Range 2 min
  • Use the Appropriate Detection Chemistry 1 min
  • Use An Appropriate Imaging Platform and Analysis Software 2 min
  • Include Biological and Technical Replicates 2 min
  • Test Your Knowledge
  • Return to the Homepage