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Normalization Strategies

Normalization Strategies

There are several things to consider before beginning your experiments. Learn more about the importance of normalization and how it can be done using three different strategies.

About this course

In this course, you will learn what normalization is and how it works using three different strategies. You will learn how to evaluate and determine the best normalization approach for your experimental conditions and context, as well as what types of variation normalization can correct for. The mechanics of how to apply normalization to data sets will also be covered.

Course Release Date: April 4, 2018

Curriculum52 min

  • Normalization Overview
  • Connection Between Journal Requirements and Normalization 2 min
  • Biological and Technical Variation 4 min
  • Attributes of an Internal Loading Control 4 min
  • Normalization Methods
  • Housekeeping Proteins 6 min
  • Signal Protein Strategy 4 min
  • Total Protein Staining 4 min
  • Pre-Immunodetection Membrane Staining 4 min
  • Choose Your Strategy 3 min
  • Normalization Analysis Examples
  • Normalization Using Housekeeping Proteins 8 min
  • Normalization Using REVERT Total Protein Stain 6 min
  • Normalization Using the Signal Protein Strategy (Post-Translational Modification) 7 min
  • Calculation Exercises
  • Normalization Exercises
  • Return to the Homepage

About this course

In this course, you will learn what normalization is and how it works using three different strategies. You will learn how to evaluate and determine the best normalization approach for your experimental conditions and context, as well as what types of variation normalization can correct for. The mechanics of how to apply normalization to data sets will also be covered.

Course Release Date: April 4, 2018

Curriculum52 min

  • Normalization Overview
  • Connection Between Journal Requirements and Normalization 2 min
  • Biological and Technical Variation 4 min
  • Attributes of an Internal Loading Control 4 min
  • Normalization Methods
  • Housekeeping Proteins 6 min
  • Signal Protein Strategy 4 min
  • Total Protein Staining 4 min
  • Pre-Immunodetection Membrane Staining 4 min
  • Choose Your Strategy 3 min
  • Normalization Analysis Examples
  • Normalization Using Housekeeping Proteins 8 min
  • Normalization Using REVERT Total Protein Stain 6 min
  • Normalization Using the Signal Protein Strategy (Post-Translational Modification) 7 min
  • Calculation Exercises
  • Normalization Exercises
  • Return to the Homepage