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Quantitative Western Blot Analysis with Replicate Samples

Quantitative Western Blot Analysis with Replicate Samples

Replicate samples are essential to quantitative Western blotting. Learn more about technical versus biological replicates, and how to incorporate replicates into your experiments.

About this course

In this course, you will learn about the two types of replicate samples, biological and technical, and what questions about your experiment each addresses. You will learn how to proceed, what samples and controls will be needed, and the number and types of replicates required. You will also learn how to use ratiometric analysis to determine fold change or percent change so that you can accurately determine changes in the relative abundance of a target protein and to evaluate relative protein levels across a group of samples in your quantitative Western blot.

Course Release Date: April 4, 2018

Curriculum20 min

  • Types of Replicates
  • Biological Replicates 2 min
  • Technical Replicates 2 min
  • Important Components of Replicates
  • Sample Size 1 min
  • Goal of a Quantitative Western Blot 2 min
  • Calculating Fold Change 2 min
  • Calculating Percent Change 1 min
  • Example of Replicates Experiment
  • Planning a Replicate Experiment 2 min
  • Performing a Replicate Experiment 5 min
  • Best Practices
  • Best Practices: Explained 3 min
  • Return to the Homepage

About this course

In this course, you will learn about the two types of replicate samples, biological and technical, and what questions about your experiment each addresses. You will learn how to proceed, what samples and controls will be needed, and the number and types of replicates required. You will also learn how to use ratiometric analysis to determine fold change or percent change so that you can accurately determine changes in the relative abundance of a target protein and to evaluate relative protein levels across a group of samples in your quantitative Western blot.

Course Release Date: April 4, 2018

Curriculum20 min

  • Types of Replicates
  • Biological Replicates 2 min
  • Technical Replicates 2 min
  • Important Components of Replicates
  • Sample Size 1 min
  • Goal of a Quantitative Western Blot 2 min
  • Calculating Fold Change 2 min
  • Calculating Percent Change 1 min
  • Example of Replicates Experiment
  • Planning a Replicate Experiment 2 min
  • Performing a Replicate Experiment 5 min
  • Best Practices
  • Best Practices: Explained 3 min
  • Return to the Homepage