Transferring the Proteins

Transferring the Proteins

Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.

About this course

Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.

Curriculum34 min

  • Science
  • Types of Transfer and Potential for Variability 4 min
  • Transfer Components and Current/Voltage Specifications 4 min
  • Best Practices
  • Optimize Transfer Conditions 7 min
  • Optimize Buffer Composition 5 min
  • Confirm Transfer Efficiency 5 min
  • Work with Clean Transfer Pads 4 min
  • In the Lab
  • Transferring the Proteins 5 min
  • Knowledge Check
  • Return to the Homepage

About this course

Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.

Curriculum34 min

  • Science
  • Types of Transfer and Potential for Variability 4 min
  • Transfer Components and Current/Voltage Specifications 4 min
  • Best Practices
  • Optimize Transfer Conditions 7 min
  • Optimize Buffer Composition 5 min
  • Confirm Transfer Efficiency 5 min
  • Work with Clean Transfer Pads 4 min
  • In the Lab
  • Transferring the Proteins 5 min
  • Knowledge Check
  • Return to the Homepage