NIR: Detection

NIR: Detection

There are several things you need to keep in mind when imaging an NIR Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative.

About this course

There are several things you need to keep in mind when imaging an NIR Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative. 

Curriculum44 min

  • Science
  • Light Source and Detector Options 7 min
  • Stripping the Membrane 3 min
  • Re-probing and Re-imaging 2 min
  • Troubleshooting 9 min
  • Best Practices
  • Imaging a Wet vs. Dry Membrane 5 min
  • Image Manipulations and Storage Considerations 4 min
  • Stripping Your Membrane 3 min
  • Reprobing Your Membrane 2 min
  • Troubleshooting Your Membrane 3 min
  • General Best Practices 4 min
  • In the Lab
  • Detection 2 min
  • Knowledge Check
  • Return to the Homepage

About this course

There are several things you need to keep in mind when imaging an NIR Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative. 

Curriculum44 min

  • Science
  • Light Source and Detector Options 7 min
  • Stripping the Membrane 3 min
  • Re-probing and Re-imaging 2 min
  • Troubleshooting 9 min
  • Best Practices
  • Imaging a Wet vs. Dry Membrane 5 min
  • Image Manipulations and Storage Considerations 4 min
  • Stripping Your Membrane 3 min
  • Reprobing Your Membrane 2 min
  • Troubleshooting Your Membrane 3 min
  • General Best Practices 4 min
  • In the Lab
  • Detection 2 min
  • Knowledge Check
  • Return to the Homepage