On-Demand Western Blot Education Portal

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Consistency in lab techniques and procedures is crucial for reproducible research results.

Lambda U® courses are designed for brand new students, seasoned pros who need a refresher, and everyone in between. Modular course designs allow you to learn what you need and get to work.

Dilutions and Calculations
Dilutions and Calculations
Check this course out for a refresher on the basics of dilutions and other calculations needed for Western blotting.
FREE
16 min
Laboratory Techniques
Blocking Step Through Image Adjustment for NIR
Blocking Step Through Image Adjustment for NIR
Learn about the basics of Near-Infrared Western blotting, including blocking the membrane, antibody selection, and more.
FREE
25 min
Introduction to Western Blotting
Introduction to Quantitative Western Blotting (FREE)
Introduction to Quantitative Western Blotting (FREE)
Learn what a quantitative Western blot is and is not, and how it differs from a qualitative Western blot.
FREE
19 min
Western BlottingQuantitative Westerns
Quantitative Western Blot Analysis with Replicate Samples
Quantitative Western Blot Analysis with Replicate Samples
Replicate samples are essential to quantitative Western blotting. Learn more about technical versus biological replicates, and how to incorporate replicates into your experiments.
$99
20 min
ReplicatesWestern Blot
Publishing Western Blot Data
Publishing Western Blot Data
Learn more about the things to consider when working towards publishing your research, including the peer review process and the importance of choosing the right journal.
$99
21 min
Western BlotPublishing
Data Analysis and Basic Quantification
Data Analysis and Basic Quantification
Proper data analysis and quantification are essential when drawing meaningful conclusions from your data. Improving in these areas will have a significant impact on your research.
$99
54 min
Data AnalysisData QuantificationWestern Blotting
Linear Range for Quantitative Western Blots
Linear Range for Quantitative Western Blots
Learn more about what linear range and dynamic range are, why they are important, and how they play a significant role in getting high quality data that you can count on.
$99
25 min
Western BlottingLinear Range
Normalization Strategies
Normalization Strategies
There are several things to consider before beginning your experiments. Learn more about the importance of normalization and how it can be done using three different strategies.
$99
52 min
Western BlottingNormalization
Antibody Validation
Antibody Validation
Antibody validation is an important step in the Western blot process. Learn more about how specificity, selectivity, and reproducibility impact your antibody choice.
$99
28 min
Western BlottingAntibodiesValidation
Antibody Ins and Outs
Antibody Ins and Outs
Learn about the importance of antibodies and how their different qualities can impact your experiments.
$59
24 min
Quantitative Western Blotting 101AntibodiesQuantitative Westerns
Antibody Selection
Antibody Selection
Antibody selection is an important step in ensuring the antibodies in your experiment give you reliable results.
$59
24 min
Western BlottingAntibodies
Estimating Protein Concentration
Estimating Protein Concentration
Learn about the importance of estimating your protein concentration and how to incorporate this technique into your experimental design.
$59
38 min
Protein Concentration
BCA Workflow
BCA Workflow
The BCA assay is one of the most popular protein estimation assays. Learn how to perform this assay and how you can integrate it into your workflow.
$59
25 min
Protein ConcentrationBCA Assay
What is a Western Blot?
What is a Western Blot?
Western blots are used a several different areas of life science research. This course will dive into the process of a Western blot, as well as why and when you would choose to use that technique.
FREE
18 min
Introduction to Western BlottingWestern BlotWestern Blotting
Preparing the Samples
Preparing the Samples
Your Western blot can only be as good as the samples you start with. Learn more about the things to consider when preparing your samples and how to minimize the amount of variability that gets added to your experiment.
FREE
27 min
Introduction to Western BlottingWestern BlotWestern BlottingSample Preparation
Preparing the Gel
Preparing the Gel
It is important to take your targets into consideration when selecting the gel type and percentage, as well as buffer composition when running and SDS-PAGE. This will help ensure that you have an even run of your samples, which will impact the accuracy and robustness of your data.
FREE
28 min
Introduction to Western BlottingWestern BlotWestern BlottingGel Preparation
Equilibrating the Gel and Preparing for Transfer
Equilibrating the Gel and Preparing for Transfer
Gel equilibration is a critical step to help ensure an even transfer of your proteins from the gel to the membrane. It's also important to choose the right membrane for your targets. Paying close attention in these details will help increase the accuracy and robustness of your data downstream.
FREE
21 min
Introduction to Western BlottingPreparing for TransferEquilibrating the Gel
Transferring the Proteins
Transferring the Proteins
Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.
FREE
34 min
Introduction to Western BlottingProtein Transfer
Drying the Membrane
Drying the Membrane
Drying the membrane may sound odd, but it is actually better for your Western blot data in the long run. In this course, we will discuss why you should dry your membrane, and how to accomplish that to leave you with a great Western blot membrane.
FREE
13 min
Introduction to Western BlottingDrying the membrane
Chemiluminescence: Blocking the Membrane
Chemiluminescence: Blocking the Membrane
Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.
FREE
40 min
Western BlotWestern BlottingQuantitative WesternsBlocking
Chemiluminescence: Primary Antibody Incubation
Chemiluminescence: Primary Antibody Incubation
Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots.
FREE
29 min
Western BlotWestern BlottingAntibodiesQuantitative WesternsPrimary Antibody
Secondary Antibody Incubation Through Image Adjustment for Chemiluminescence
Secondary Antibody Incubation Through Image Adjustment for Chemiluminescence
Learn about the basics of chemiluminescent Western blotting, including antibody selection, detection, and more.
FREE
14 min
Western BlotWestern BlottingAntibodies
Introduction to Western Blotting
Introduction to Western Blotting
10 Courses
Introduction to Western BlottingWestern BlotWestern BlottingSample PreparationGel PreparationPreparing for TransferEquilibrating the GelProtein TransferDrying the membrane
Meeting Western Blot Publication and Grant Funding Requirements
Meeting Western Blot Publication and Grant Funding Requirements
Equip yourself and your lab with valuable information and tips to meet even the toughest requirements.
$499
7 Courses
ReplicatesWestern BlotPublishingData AnalysisData QuantificationWestern BlottingLinear RangeNormalizationAntibodiesValidationQuantitative Westerns
Presentation: How to Best Present a Quantitative Western Blot for Publication
Presentation: How to Best Present a Quantitative Western Blot for Publication
Hear from Deputy Editor, F. Peter Guengerich, Ph.D, about The Journal of Biological Chemistry’s revised guidelines for presenting your quantitative Western blots and improving the reliability of research.
FREE
28 min
Publishing
Protein Concentration
Protein Concentration
Estimating your protein concentration is important for getting accurate data.
$99
2 Courses
Protein ConcentrationBCA Assay
The Importance of Antibodies
The Importance of Antibodies
Antibodies play an important role in Western blotting. Become an antibody expert.
$99
2 Courses
Quantitative Western Blotting 101Western BlottingAntibodiesQuantitative Westerns
Laboratory Techniques
Laboratory Techniques
1 Course
Laboratory Techniques