Equilibrating the Gel and Preparing for Transfer
Gel equilibration is a critical step to help ensure an even transfer of your proteins from the gel to the membrane. It's also important to choose the right membrane for your targets. Paying close attention in these details will help increase the accuracy and robustness of your data downstream.
FREE
21 min
Introduction to Western BlottingPreparing for TransferEquilibrating the Gel
Chemiluminescence: Detection
There are several things to keep in mind when using a chemiluminescent detection system. In this course, you'll learn about the importance of the substrate and how it could impact your final results, as well other considerations that will help you get consistent and robust data from your Western blo
FREE
52 min
Introduction to Western BlottingWestern BlotWestern BlottingDetection
Linear Range for Quantitative Western Blots
Learn more about what linear range and dynamic range are, why they are important, and how they play a significant role in getting high quality data that you can count on.
$99
25 min
Western BlottingLinear Range
Chemiluminescence: Blocking the Membrane
Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.
FREE
40 min
Western BlotWestern BlottingQuantitative WesternsBlocking
Chemiluminescence: Primary Antibody Incubation
Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots.
FREE
29 min
Western BlotWestern BlottingAntibodiesQuantitative WesternsPrimary Antibody
Chemiluminescence: Secondary Antibody Incubation
Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results.
FREE
14 min
Western BlotWestern BlottingAntibodiesQuantitative WesternsSecondary Antibodies
NIR: Secondary Antibody Incubation
Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results.
FREE
31 min
Western BlottingSecondary Antibodies
Transferring the Proteins
Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.
FREE
34 min
Introduction to Western BlottingProtein Transfer