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On-Demand Western Blot Education Portal

Take Steps Toward Better Data

Consistency in lab techniques and procedures is crucial for reproducible research results.

Lambda U® courses are designed for brand new students, seasoned pros who need a refresher, and everyone in between. Modular course designs allow you to learn what you need and get to work.

Introduction to Western Blotting

Introduction to Western Blotting

FREE | 15 Courses

Introduction to Western Blotting

Introduction to Western Blotting

Introduction to Western BlottingWestern BlotWestern BlottingSample PreparationGel PreparationPreparing for TransferEquilibrating the GelProtein TransferDrying the membrane

Meeting Western Blot Publication and Grant Funding Requirements

Meeting Western Blot Publication and Grant Funding Requirements

Equip yourself and your lab with valuable information and tips to meet even the toughest requirements.

ReplicatesWestern BlotPublishingData AnalysisData QuantificationWestern BlottingLinear RangeNormalizationAntibodiesValidationQuantitative Westerns

Presentation: How to Best Present a Quantitative Western Blot for Publication

Presentation: How to Best Present a Quantitative Western Blot for Publication

Hear from Deputy Editor, F. Peter Guengerich, Ph.D, about The Journal of Biological Chemistry’s revised guidelines for presenting your quantitative Western blots and improving the reliability of research.

The Importance of Antibodies

The Importance of Antibodies

Antibodies play an important role in Western blotting. Become an antibody expert.

Quantitative Western Blotting 101Western BlottingAntibodiesQuantitative Westerns

Protein Concentration

Protein Concentration

Estimating your protein concentration is important for getting accurate data.

Protein ConcentrationBCA Assay

Laboratory Techniques

Laboratory Techniques

Laboratory Techniques

Equilibrating the Gel and Preparing for Transfer

Equilibrating the Gel and Preparing for Transfer

Gel equilibration is a critical step to help ensure an even transfer of your proteins from the gel to the membrane. It's also important to choose the right membrane for your targets. Paying close attention in these details will help increase the accuracy and robustness of your data downstream.

Introduction to Western BlottingPreparing for TransferEquilibrating the Gel

Quantitative Western Blot Analysis with Replicate Samples

Quantitative Western Blot Analysis with Replicate Samples

Replicate samples are essential to quantitative Western blotting. Learn more about technical versus biological replicates, and how to incorporate replicates into your experiments.

ReplicatesWestern Blot

Antibody Ins and Outs

Antibody Ins and Outs

Learn about the importance of antibodies and how their different qualities can impact your experiments.

Quantitative Western Blotting 101AntibodiesQuantitative Westerns

Chemiluminescence: Detection

Chemiluminescence: Detection

There are several things to keep in mind when using a chemiluminescent detection system. In this course, you'll learn about the importance of the substrate and how it could impact your final results, as well other considerations that will help you get consistent and robust data from your Western blo

Introduction to Western BlottingWestern BlotWestern BlottingDetection

What is a Western Blot?

What is a Western Blot?

Western blots are used a several different areas of life science research. This course will dive into the process of a Western blot, as well as why and when you would choose to use that technique.

Introduction to Western BlottingWestern BlotWestern Blotting

NIR: Blocking the Membrane

NIR: Blocking the Membrane

NIR technology is relatively new but is a very powerful way to image Western blots. In this course, you'll learn about the blocking step in the NIR Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Western BlottingBlocking

Linear Range for Quantitative Western Blots

Linear Range for Quantitative Western Blots

Learn more about what linear range and dynamic range are, why they are important, and how they play a significant role in getting high quality data that you can count on.

Western BlottingLinear Range

Preparing the Samples

Preparing the Samples

Your Western blot can only be as good as the samples you start with. Learn more about the things to consider when preparing your samples and how to minimize the amount of variability that gets added to your experiment.

Introduction to Western BlottingWestern BlotWestern BlottingSample Preparation

Preparing the Gel

Preparing the Gel

It is important to take your targets into consideration when selecting the gel type and percentage, as well as buffer composition when running and SDS-PAGE. This will help ensure that you have an even run of your samples, which will impact the accuracy and robustness of your data.

Introduction to Western BlottingWestern BlotWestern BlottingGel Preparation

Chemiluminescence: Blocking the Membrane

Chemiluminescence: Blocking the Membrane

Chemiluminescence is a commonly used detection method for Western blotting. In this course, you'll learn about the blocking step in the chemiluminescent Western blot protocol and how you can reduce variability in your results by selecting the right blocker for your experiment.

Western BlotWestern BlottingQuantitative WesternsBlocking

Antibody Validation

Antibody Validation

Antibody validation is an important step in the Western blot process. Learn more about how specificity, selectivity, and reproducibility impact your antibody choice.

Western BlottingAntibodiesValidation

Estimating Protein Concentration

Estimating Protein Concentration

Learn about the importance of estimating your protein concentration and how to incorporate this technique into your experimental design.

Protein Concentration

Chemiluminescence: Primary Antibody Incubation

Chemiluminescence: Primary Antibody Incubation

Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots.

Western BlotWestern BlottingAntibodiesQuantitative WesternsPrimary Antibody

NIR: Primary Antibody Incubation

NIR: Primary Antibody Incubation

Learn the science behind how primary antibody incubation works, and why it is an important step in the process. You will also learn some tips that you can implement in your workflow to continue getting robust data from your blots.

Western BlottingPrimary Antibody

Dilutions and Calculations

Dilutions and Calculations

Check this course out for a refresher on the basics of dilutions and other calculations needed for Western blotting.

Laboratory Techniques

Publishing Western Blot Data

Publishing Western Blot Data

Learn more about the things to consider when working towards publishing your research, including the peer review process and the importance of choosing the right journal.

Western BlotPublishing

Normalization Strategies

Normalization Strategies

There are several things to consider before beginning your experiments. Learn more about the importance of normalization and how it can be done using three different strategies.

Western BlottingNormalization

There are several things you need to keep in mind when imaging an NIR Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative.

Western BlottingDetection

Data Analysis and Basic Quantification

Data Analysis and Basic Quantification

Proper data analysis and quantification are essential when drawing meaningful conclusions from your data. Improving in these areas will have a significant impact on your research.

Data AnalysisData QuantificationWestern Blotting

Chemiluminescence: Secondary Antibody Incubation

Chemiluminescence: Secondary Antibody Incubation

Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results.

Western BlotWestern BlottingAntibodiesQuantitative WesternsSecondary Antibodies

Introduction to Quantitative Western Blotting (FREE)

Introduction to Quantitative Western Blotting (FREE)

Learn what a quantitative Western blot is and is not, and how it differs from a qualitative Western blot.

Western BlottingQuantitative Westerns

Image Adjustments

Image Adjustments

As publishers and funding agencies revise guidelines on data submission, you need to be aware of how these changes affect the way you analyze your Western blot data. We will review some best practices and things to consider to get you started; however, be sure to review specific journal guidelines prior to data submission.

Data AnalysisData QuantificationImage adjustments

Antibody Selection

Antibody Selection

Antibody selection is an important step in ensuring the antibodies in your experiment give you reliable results.

Western BlottingAntibodies

NIR: Secondary Antibody Incubation

NIR: Secondary Antibody Incubation

Secondary antibodies can have a large impact on the quality of data that you get from your Western blots. Learn about the role that secondary antibodies play in the Western blot process, and some best practices for getting great results.

Western BlottingSecondary Antibodies

The BCA assay is one of the most popular protein estimation assays. Learn how to perform this assay and how you can integrate it into your workflow.

Protein ConcentrationBCA Assay

Transferring the Proteins

Transferring the Proteins

Protein transfer is a distinguishing step of the Western blotting protocol. It is important to consider all of the aspects of transfer including your transfer conditions, buffer, and apparatus. These have the potential to impact your transfer, and ultimately your Western blot data.

Introduction to Western BlottingProtein Transfer

Drying the Membrane

Drying the Membrane

Drying the membrane may sound odd, but it is actually better for your Western blot data in the long run. In this course, we will discuss why you should dry your membrane, and how to accomplish that to leave you with a great Western blot membrane.

Introduction to Western BlottingDrying the membrane